Construct: shRNA TRCN0000249122

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146170.3-437s21c1
DNA Barcode:
CCCGATGTGGTACGTTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C87436 (232196)
Gene Description:
expressed sequence C87436
Transcript:
RefSeq NM_146170.3 (NON-CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249122)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGATGTGGTACGTTCAATG
Hairpin Sequence:
5'-CCGG-CCCGATGTGGTACGTTCAATG-CTCGAG-CATTGAACGTACCACATCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGATGTGGTACGTTCAATGCTCGAGCATTGAACGTACCACATCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGATGTGGTACGTTCAATGCTCGAGCATTGAACGTACCACATCGGG-3'

Other clones with same target sequence:

(none)