Construct: shRNA TRCN0000249128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080640.4-291s21c1
DNA Barcode:
ATGAACTGTGGGACCCAATGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Baalc (118452)
Gene Description:
brain and acute leukemia, cytoplasmic
Transcript:
RefSeq NM_080640.4 (NON-CURRENT)
Match location:
Position 291 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAACTGTGGGACCCAATGT
Hairpin Sequence:
5'-CCGG-ATGAACTGTGGGACCCAATGT-CTCGAG-ACATTGGGTCCCACAGTTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAACTGTGGGACCCAATGTCTCGAGACATTGGGTCCCACAGTTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAACTGTGGGACCCAATGTCTCGAGACATTGGGTCCCACAGTTCAT-3'

Other clones with same target sequence:

(none)