Construct: shRNA TRCN0000249176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029454.4-1807s21c1
DNA Barcode:
CACGTCCAGAGACCATTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pibf1 (52023)
Gene Description:
progesterone immunomodulatory binding factor 1
Transcript:
RefSeq NM_029454.4 (NON-CURRENT)
Match location:
Position 1807 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGTCCAGAGACCATTATAA
Hairpin Sequence:
5'-CCGG-CACGTCCAGAGACCATTATAA-CTCGAG-TTATAATGGTCTCTGGACGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGTCCAGAGACCATTATAACTCGAGTTATAATGGTCTCTGGACGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGTCCAGAGACCATTATAACTCGAGTTATAATGGTCTCTGGACGTG-3'

Other clones with same target sequence:

(none)