Construct: shRNA TRCN0000249192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178594.2-510s21c1
DNA Barcode:
GAGATAAATGTGGACTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vtcn1 (242122)
Gene Description:
V-set domain containing T cell activation inhibitor 1
Transcript:
RefSeq NM_178594.2 (NON-CURRENT)
Match location:
Position 510 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATAAATGTGGACTATAAT
Hairpin Sequence:
5'-CCGG-GAGATAAATGTGGACTATAAT-CTCGAG-ATTATAGTCCACATTTATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATAAATGTGGACTATAATCTCGAGATTATAGTCCACATTTATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATAAATGTGGACTATAATCTCGAGATTATAGTCCACATTTATCTC-3'

Other clones with same target sequence:

(none)