Construct: shRNA TRCN0000249201

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081291.1-4711s21c1
DNA Barcode:
GGGAGATGATTGGTCCAAACC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc88b (78317)
Gene Description:
coiled-coil domain containing 88B
Transcript:
RefSeq NM_001081291.1 (CURRENT)
Match location:
Position 4711 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249201)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAGATGATTGGTCCAAACC
Hairpin Sequence:
5'-CCGG-GGGAGATGATTGGTCCAAACC-CTCGAG-GGTTTGGACCAATCATCTCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAGATGATTGGTCCAAACCCTCGAGGGTTTGGACCAATCATCTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAGATGATTGGTCCAAACCCTCGAGGGTTTGGACCAATCATCTCCC-3'

Other clones with same target sequence:

(none)