Construct: shRNA TRCN0000249221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026983.2-713s21c1
DNA Barcode:
CTGCCAGCTCTGGCCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erp27 (69187)
Gene Description:
endoplasmic reticulum protein 27
Transcript:
RefSeq NM_026983.2 (CURRENT)
Match location:
Position 713 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCCAGCTCTGGCCATTTAT
Hairpin Sequence:
5'-CCGG-CTGCCAGCTCTGGCCATTTAT-CTCGAG-ATAAATGGCCAGAGCTGGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCCAGCTCTGGCCATTTATCTCGAGATAAATGGCCAGAGCTGGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCCAGCTCTGGCCATTTATCTCGAGATAAATGGCCAGAGCTGGCAG-3'

Other clones with same target sequence:

(none)