Construct: shRNA TRCN0000249247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181590.4-215s21c1
DNA Barcode:
TCTACGCCAAGCCGTACTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shq1 (72171)
Gene Description:
SHQ1 homolog (S. cerevisiae)
Transcript:
RefSeq NM_181590.4 (NON-CURRENT)
Match location:
Position 215 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTACGCCAAGCCGTACTTTC
Hairpin Sequence:
5'-CCGG-TCTACGCCAAGCCGTACTTTC-CTCGAG-GAAAGTACGGCTTGGCGTAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACGCCAAGCCGTACTTTCCTCGAGGAAAGTACGGCTTGGCGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACGCCAAGCCGTACTTTCCTCGAGGAAAGTACGGCTTGGCGTAGA-3'

Other clones with same target sequence:

(none)