Construct: shRNA TRCN0000249277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153594.2-394s21c1
DNA Barcode:
CTATTGATCGAGCAGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcmtd2 (245867)
Gene Description:
protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2
Transcript:
RefSeq NM_153594.2 (NON-CURRENT)
Match location:
Position 394 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATTGATCGAGCAGATTATT
Hairpin Sequence:
5'-CCGG-CTATTGATCGAGCAGATTATT-CTCGAG-AATAATCTGCTCGATCAATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATTGATCGAGCAGATTATTCTCGAGAATAATCTGCTCGATCAATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATTGATCGAGCAGATTATTCTCGAGAATAATCTGCTCGATCAATAG-3'

Other clones with same target sequence:

(none)