Construct: shRNA TRCN0000249291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026420.2-282s21c1
DNA Barcode:
GAATTTATTGAACGCTGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Paip2 (67869)
Gene Description:
polyadenylate-binding protein-interacting protein 2
Transcript:
RefSeq NM_026420.2 (CURRENT)
Match location:
Position 282 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTTATTGAACGCTGTTTC
Hairpin Sequence:
5'-CCGG-GAATTTATTGAACGCTGTTTC-CTCGAG-GAAACAGCGTTCAATAAATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTTATTGAACGCTGTTTCCTCGAGGAAACAGCGTTCAATAAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTTATTGAACGCTGTTTCCTCGAGGAAACAGCGTTCAATAAATTC-3'

Other clones with same target sequence:

  1. TRCN0000434340