Construct: shRNA TRCN0000249311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177574.3-146s21c1
DNA Barcode:
CTCCGGGATCTGCTTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps37d (194309)
Gene Description:
vacuolar protein sorting 37D
Transcript:
RefSeq NM_177574.3 (NON-CURRENT)
Match location:
Position 146 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCGGGATCTGCTTCAAGAT
Hairpin Sequence:
5'-CCGG-CTCCGGGATCTGCTTCAAGAT-CTCGAG-ATCTTGAAGCAGATCCCGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCGGGATCTGCTTCAAGATCTCGAGATCTTGAAGCAGATCCCGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCGGGATCTGCTTCAAGATCTCGAGATCTTGAAGCAGATCCCGGAG-3'

Other clones with same target sequence:

(none)