Construct: shRNA TRCN0000249312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177574.3-988s21c1
DNA Barcode:
CTTACTGGATTGTTCTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps37d (194309)
Gene Description:
vacuolar protein sorting 37D
Transcript:
RefSeq NM_177574.3 (NON-CURRENT)
Match location:
Position 988 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACTGGATTGTTCTCATAT
Hairpin Sequence:
5'-CCGG-CTTACTGGATTGTTCTCATAT-CTCGAG-ATATGAGAACAATCCAGTAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACTGGATTGTTCTCATATCTCGAGATATGAGAACAATCCAGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACTGGATTGTTCTCATATCTCGAGATATGAGAACAATCCAGTAAG-3'

Other clones with same target sequence:

(none)