Construct: shRNA TRCN0000249360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026599.4-254s21c1
DNA Barcode:
ATTGATGGCCACCCTTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cgnl1 (68178)
Gene Description:
cingulin-like 1
Transcript:
RefSeq NM_026599.4 (NON-CURRENT)
Match location:
Position 254 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTGATGGCCACCCTTATATA
Hairpin Sequence:
5'-CCGG-ATTGATGGCCACCCTTATATA-CTCGAG-TATATAAGGGTGGCCATCAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTGATGGCCACCCTTATATACTCGAGTATATAAGGGTGGCCATCAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTGATGGCCACCCTTATATACTCGAGTATATAAGGGTGGCCATCAAT-3'

Other clones with same target sequence:

(none)