Construct: shRNA TRCN0000249361

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026599.4-5921s21c1
DNA Barcode:
TCATGTCATCATTCGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cgnl1 (68178)
Gene Description:
cingulin-like 1
Transcript:
RefSeq NM_026599.4 (NON-CURRENT)
Match location:
Position 5921 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249361)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATGTCATCATTCGATATTT
Hairpin Sequence:
5'-CCGG-TCATGTCATCATTCGATATTT-CTCGAG-AAATATCGAATGATGACATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATGTCATCATTCGATATTTCTCGAGAAATATCGAATGATGACATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATGTCATCATTCGATATTTCTCGAGAAATATCGAATGATGACATGA-3'

Other clones with same target sequence:

(none)