Construct: shRNA TRCN0000249395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145939.1-244s21c1
DNA Barcode:
GGTTTCATCAATGGCACATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alg3 (208624)
Gene Description:
asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase)
Transcript:
RefSeq NM_145939.1 (NON-CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTTCATCAATGGCACATAT
Hairpin Sequence:
5'-CCGG-GGTTTCATCAATGGCACATAT-CTCGAG-ATATGTGCCATTGATGAAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTTCATCAATGGCACATATCTCGAGATATGTGCCATTGATGAAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTTCATCAATGGCACATATCTCGAGATATGTGCCATTGATGAAACC-3'

Other clones with same target sequence:

(none)