Construct: shRNA TRCN0000249508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026822.1-364s21c1
DNA Barcode:
TGTAGCAGTGGTGGCAGCAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lce1b (68720)
Gene Description:
late cornified envelope 1B
Transcript:
RefSeq NM_026822.1 (CURRENT)
Match location:
Position 364 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAGCAGTGGTGGCAGCAGT
Hairpin Sequence:
5'-CCGG-TGTAGCAGTGGTGGCAGCAGT-CTCGAG-ACTGCTGCCACCACTGCTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAGCAGTGGTGGCAGCAGTCTCGAGACTGCTGCCACCACTGCTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAGCAGTGGTGGCAGCAGTCTCGAGACTGCTGCCACCACTGCTACA-3'

Other clones with same target sequence:

(none)