Construct: shRNA TRCN0000249520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013603.1-139s21c1
DNA Barcode:
CGAACTGCAAGAAGAGCTGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mt3 (17751)
Gene Description:
metallothionein 3
Transcript:
RefSeq NM_013603.1 (NON-CURRENT)
Match location:
Position 139 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAACTGCAAGAAGAGCTGCT
Hairpin Sequence:
5'-CCGG-CGAACTGCAAGAAGAGCTGCT-CTCGAG-AGCAGCTCTTCTTGCAGTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAACTGCAAGAAGAGCTGCTCTCGAGAGCAGCTCTTCTTGCAGTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAACTGCAAGAAGAGCTGCTCTCGAGAGCAGCTCTTCTTGCAGTTCG-3'

Other clones with same target sequence:

(none)