Construct: shRNA TRCN0000249521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173444.2-6380s21c1
DNA Barcode:
ATGGCAGGACGAACCTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nbeal1 (269198)
Gene Description:
neurobeachin like 1
Transcript:
RefSeq NM_173444.2 (CURRENT)
Match location:
Position 6380 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGCAGGACGAACCTATAAT
Hairpin Sequence:
5'-CCGG-ATGGCAGGACGAACCTATAAT-CTCGAG-ATTATAGGTTCGTCCTGCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGCAGGACGAACCTATAATCTCGAGATTATAGGTTCGTCCTGCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGCAGGACGAACCTATAATCTCGAGATTATAGGTTCGTCCTGCCAT-3'

Other clones with same target sequence:

  1. TRCN0000282182