Construct: shRNA TRCN0000249524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173444.2-9350s21c1
DNA Barcode:
ACCCGGATGATTACGAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nbeal1 (269198)
Gene Description:
neurobeachin like 1
Transcript:
RefSeq NM_173444.2 (CURRENT)
Match location:
Position 9350 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCGGATGATTACGAGTTAT
Hairpin Sequence:
5'-CCGG-ACCCGGATGATTACGAGTTAT-CTCGAG-ATAACTCGTAATCATCCGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCGGATGATTACGAGTTATCTCGAGATAACTCGTAATCATCCGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCGGATGATTACGAGTTATCTCGAGATAACTCGTAATCATCCGGGT-3'

Other clones with same target sequence:

(none)