Construct: shRNA TRCN0000249581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027810.3-355s21c1
DNA Barcode:
TTGCTGCAGGGTCCGAGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bbs7 (71492)
Gene Description:
Bardet-Biedl syndrome 7 (human)
Transcript:
RefSeq NM_027810.3 (CURRENT)
Match location:
Position 355 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCTGCAGGGTCCGAGATTA
Hairpin Sequence:
5'-CCGG-TTGCTGCAGGGTCCGAGATTA-CTCGAG-TAATCTCGGACCCTGCAGCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCTGCAGGGTCCGAGATTACTCGAGTAATCTCGGACCCTGCAGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCTGCAGGGTCCGAGATTACTCGAGTAATCTCGGACCCTGCAGCAA-3'

Other clones with same target sequence:

(none)