Construct: shRNA TRCN0000249613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177171.4-5028s21c1
DNA Barcode:
ATGTACTACACCGAGTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Heatr5a (320487)
Gene Description:
HEAT repeat containing 5A
Transcript:
RefSeq NM_177171.4 (CURRENT)
Match location:
Position 5028 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTACTACACCGAGTAATTT
Hairpin Sequence:
5'-CCGG-ATGTACTACACCGAGTAATTT-CTCGAG-AAATTACTCGGTGTAGTACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTACTACACCGAGTAATTTCTCGAGAAATTACTCGGTGTAGTACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTACTACACCGAGTAATTTCTCGAGAAATTACTCGGTGTAGTACAT-3'

Other clones with same target sequence:

(none)