Construct: shRNA TRCN0000249649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026613.4-1086s21c1
DNA Barcode:
GTCATCCCTGGCAGTTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc34 (68201)
Gene Description:
coiled-coil domain containing 34
Transcript:
RefSeq NM_026613.4 (CURRENT)
Match location:
Position 1086 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATCCCTGGCAGTTCATAA
Hairpin Sequence:
5'-CCGG-GTCATCCCTGGCAGTTCATAA-CTCGAG-TTATGAACTGCCAGGGATGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATCCCTGGCAGTTCATAACTCGAGTTATGAACTGCCAGGGATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATCCCTGGCAGTTCATAACTCGAGTTATGAACTGCCAGGGATGAC-3'

Other clones with same target sequence:

(none)