Construct: shRNA TRCN0000249699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033616.3-500s21c1
DNA Barcode:
TGCTGTGTTGGCTGGTATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csprs (114564)
Gene Description:
component of Sp100-rs
Transcript:
RefSeq NM_033616.3 (CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGTGTTGGCTGGTATTTG
Hairpin Sequence:
5'-CCGG-TGCTGTGTTGGCTGGTATTTG-CTCGAG-CAAATACCAGCCAACACAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGTGTTGGCTGGTATTTGCTCGAGCAAATACCAGCCAACACAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGTGTTGGCTGGTATTTGCTCGAGCAAATACCAGCCAACACAGCA-3'

Other clones with same target sequence:

(none)