Construct: shRNA TRCN0000249823

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175751.3-319s21c1
DNA Barcode:
CCAAATACAGTTGATACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp608 (269023)
Gene Description:
zinc finger protein 608
Transcript:
RefSeq NM_175751.3 (NON-CURRENT)
Match location:
Position 319 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249823)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATACAGTTGATACTTAT
Hairpin Sequence:
5'-CCGG-CCAAATACAGTTGATACTTAT-CTCGAG-ATAAGTATCAACTGTATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATACAGTTGATACTTATCTCGAGATAAGTATCAACTGTATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATACAGTTGATACTTATCTCGAGATAAGTATCAACTGTATTTGG-3'

Other clones with same target sequence:

(none)