Construct: shRNA TRCN0000249979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027759.2-1104s21c1
DNA Barcode:
GGTTCCAGGAGAAGGCTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fsip1 (71313)
Gene Description:
fibrous sheath-interacting protein 1
Transcript:
RefSeq NM_027759.2 (NON-CURRENT)
Match location:
Position 1104 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTCCAGGAGAAGGCTATAC
Hairpin Sequence:
5'-CCGG-GGTTCCAGGAGAAGGCTATAC-CTCGAG-GTATAGCCTTCTCCTGGAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTCCAGGAGAAGGCTATACCTCGAGGTATAGCCTTCTCCTGGAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTCCAGGAGAAGGCTATACCTCGAGGTATAGCCTTCTCCTGGAACC-3'

Other clones with same target sequence:

(none)