Construct: shRNA TRCN0000249999
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_177815.3-1979s21c1
- DNA Barcode:
- GCCGCAAGAATGGTTAGTTTC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rft1 (328370)
- Gene Description:
- RFT1 homolog
- Transcript:
- RefSeq NM_177815.3 (CURRENT)
- Match location:
- Position 1979 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000249999)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCGCAAGAATGGTTAGTTTC
- Hairpin Sequence:
-
5'-CCGG-GCCGCAAGAATGGTTAGTTTC-CTCGAG-GAAACTAACCATTCTTGCGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCGCAAGAATGGTTAGTTTCCTCGAGGAAACTAACCATTCTTGCGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCGCAAGAATGGTTAGTTTCCTCGAGGAAACTAACCATTCTTGCGGC-3'
Other clones with same target sequence:
(none)
