Construct: shRNA TRCN0000250023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025706.2-2065s21c1
DNA Barcode:
TTAACACCTGCATGATCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d15 (66687)
Gene Description:
TBC1 domain family, member 15
Transcript:
RefSeq NM_025706.2 (NON-CURRENT)
Match location:
Position 2065 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAACACCTGCATGATCATTT
Hairpin Sequence:
5'-CCGG-TTAACACCTGCATGATCATTT-CTCGAG-AAATGATCATGCAGGTGTTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAACACCTGCATGATCATTTCTCGAGAAATGATCATGCAGGTGTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAACACCTGCATGATCATTTCTCGAGAAATGATCATGCAGGTGTTAA-3'

Other clones with same target sequence:

(none)