Construct: shRNA TRCN0000250031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153391.2-3338s21c1
DNA Barcode:
CCGCTTGTACATGGCGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr19 (213081)
Gene Description:
WD repeat domain 19
Transcript:
RefSeq NM_153391.2 (CURRENT)
Match location:
Position 3338 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTGTACATGGCGCTAAA
Hairpin Sequence:
5'-CCGG-CCGCTTGTACATGGCGCTAAA-CTCGAG-TTTAGCGCCATGTACAAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTGTACATGGCGCTAAACTCGAGTTTAGCGCCATGTACAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTGTACATGGCGCTAAACTCGAGTTTAGCGCCATGTACAAGCGG-3'

Other clones with same target sequence:

(none)