Construct: shRNA TRCN0000250067

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146047.1-1701s21c1
DNA Barcode:
CACCCTCTCTTCTCGTGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clptm1l (218335)
Gene Description:
CLPTM1-like
Transcript:
RefSeq NM_146047.1 (NON-CURRENT)
Match location:
Position 1701 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250067)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCCTCTCTTCTCGTGTTTC
Hairpin Sequence:
5'-CCGG-CACCCTCTCTTCTCGTGTTTC-CTCGAG-GAAACACGAGAAGAGAGGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCCTCTCTTCTCGTGTTTCCTCGAGGAAACACGAGAAGAGAGGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCCTCTCTTCTCGTGTTTCCTCGAGGAAACACGAGAAGAGAGGGTG-3'

Other clones with same target sequence:

(none)