Construct: shRNA TRCN0000250071

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026157.2-2102s21c1
DNA Barcode:
TAGTACTCTCTAGTATCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtpap (67440)
Gene Description:
mitochondrial poly(A) polymerase
Transcript:
RefSeq NM_026157.2 (CURRENT)
Match location:
Position 2102 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250071)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTACTCTCTAGTATCTATT
Hairpin Sequence:
5'-CCGG-TAGTACTCTCTAGTATCTATT-CTCGAG-AATAGATACTAGAGAGTACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTACTCTCTAGTATCTATTCTCGAGAATAGATACTAGAGAGTACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTACTCTCTAGTATCTATTCTCGAGAATAGATACTAGAGAGTACTA-3'

Other clones with same target sequence:

(none)