Construct: shRNA TRCN0000250081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026283.2-1386s21c1
DNA Barcode:
AGAGTAGGAGAGCGAGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Samd8 (67630)
Gene Description:
sterile alpha motif domain containing 8
Transcript:
RefSeq NM_026283.2 (CURRENT)
Match location:
Position 1386 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTAGGAGAGCGAGGATTT
Hairpin Sequence:
5'-CCGG-AGAGTAGGAGAGCGAGGATTT-CTCGAG-AAATCCTCGCTCTCCTACTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTAGGAGAGCGAGGATTTCTCGAGAAATCCTCGCTCTCCTACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTAGGAGAGCGAGGATTTCTCGAGAAATCCTCGCTCTCCTACTCT-3'

Other clones with same target sequence:

(none)