Construct: shRNA TRCN0000250097

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001476.1-143s21c1
DNA Barcode:
CTGAAGTGATTGGGCCTATGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE6 (2578)
Gene Description:
G antigen 6
Transcript:
RefSeq NM_001476.1 (CURRENT)
Match location:
Position 143 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250097)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAAGTGATTGGGCCTATGC
Hairpin Sequence:
5'-CCGG-CTGAAGTGATTGGGCCTATGC-CTCGAG-GCATAGGCCCAATCACTTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAAGTGATTGGGCCTATGCCTCGAGGCATAGGCCCAATCACTTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAAGTGATTGGGCCTATGCCTCGAGGCATAGGCCCAATCACTTCAG-3'

Other clones with same target sequence:

(none)