Construct: shRNA TRCN0000250144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013241.2-1098s21c1
DNA Barcode:
TCTACGAGAACGCCCTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FHOD1 (29109)
Gene Description:
formin homology 2 domain containing 1
Transcript:
RefSeq NM_013241.2 (NON-CURRENT)
Match location:
Position 1098 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTACGAGAACGCCCTGAAAT
Hairpin Sequence:
5'-CCGG-TCTACGAGAACGCCCTGAAAT-CTCGAG-ATTTCAGGGCGTTCTCGTAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACGAGAACGCCCTGAAATCTCGAGATTTCAGGGCGTTCTCGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACGAGAACGCCCTGAAATCTCGAGATTTCAGGGCGTTCTCGTAGA-3'

Other clones with same target sequence:

(none)