Construct: shRNA TRCN0000250171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207547.1-450s21c1
DNA Barcode:
TAACATCCACATTCCAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r93 (404290)
Gene Description:
vomeronasal 1 receptor 93
Transcript:
RefSeq NM_207547.1 (NON-CURRENT)
Match location:
Position 450 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACATCCACATTCCAATTAA
Hairpin Sequence:
5'-CCGG-TAACATCCACATTCCAATTAA-CTCGAG-TTAATTGGAATGTGGATGTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACATCCACATTCCAATTAACTCGAGTTAATTGGAATGTGGATGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACATCCACATTCCAATTAACTCGAGTTAATTGGAATGTGGATGTTA-3'

Other clones with same target sequence:

(none)