Construct: shRNA TRCN0000250178

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198292.3-2839s21c1
DNA Barcode:
ACTTGACCAAACTAGGTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tex2 (21763)
Gene Description:
testis expressed gene 2
Transcript:
RefSeq NM_198292.3 (CURRENT)
Match location:
Position 2839 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250178)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGACCAAACTAGGTAAAG
Hairpin Sequence:
5'-CCGG-ACTTGACCAAACTAGGTAAAG-CTCGAG-CTTTACCTAGTTTGGTCAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGACCAAACTAGGTAAAGCTCGAGCTTTACCTAGTTTGGTCAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGACCAAACTAGGTAAAGCTCGAGCTTTACCTAGTTTGGTCAAGT-3'

Other clones with same target sequence:

(none)