Construct: shRNA TRCN0000250289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207522.1-566s21c1
DNA Barcode:
TTCTGGTAAAGGCTATCTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl25 (216292)
Gene Description:
methyltransferase like 25
Transcript:
RefSeq NM_207522.1 (NON-CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTGGTAAAGGCTATCTAAG
Hairpin Sequence:
5'-CCGG-TTCTGGTAAAGGCTATCTAAG-CTCGAG-CTTAGATAGCCTTTACCAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTGGTAAAGGCTATCTAAGCTCGAGCTTAGATAGCCTTTACCAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTGGTAAAGGCTATCTAAGCTCGAGCTTAGATAGCCTTTACCAGAA-3'

Other clones with same target sequence:

(none)