Construct: shRNA TRCN0000250353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015773.1-1345s21c1
DNA Barcode:
TACGACGCACCTCCCAATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spag6l (50525)
Gene Description:
sperm associated antigen 6-like
Transcript:
RefSeq NM_015773.1 (NON-CURRENT)
Match location:
Position 1345 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGACGCACCTCCCAATATC
Hairpin Sequence:
5'-CCGG-TACGACGCACCTCCCAATATC-CTCGAG-GATATTGGGAGGTGCGTCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGACGCACCTCCCAATATCCTCGAGGATATTGGGAGGTGCGTCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGACGCACCTCCCAATATCCTCGAGGATATTGGGAGGTGCGTCGTA-3'

Other clones with same target sequence:

(none)