Construct: shRNA TRCN0000250449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133771.1-291s21c1
DNA Barcode:
AGACCTGCTAGAGCCATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Memo1 (76890)
Gene Description:
mediator of cell motility 1
Transcript:
RefSeq NM_133771.1 (NON-CURRENT)
Match location:
Position 291 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCTGCTAGAGCCATTATT
Hairpin Sequence:
5'-CCGG-AGACCTGCTAGAGCCATTATT-CTCGAG-AATAATGGCTCTAGCAGGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCTGCTAGAGCCATTATTCTCGAGAATAATGGCTCTAGCAGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCTGCTAGAGCCATTATTCTCGAGAATAATGGCTCTAGCAGGTCT-3'

Other clones with same target sequence:

(none)