Construct: shRNA TRCN0000250495
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_145981.3-2423s21c1
- DNA Barcode:
- GGGATTGTCATAGTAACATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Phyhip (105653)
- Gene Description:
- phytanoyl-CoA hydroxylase interacting protein
- Transcript:
- RefSeq NM_145981.3 (CURRENT)
- Match location:
- Position 2423 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000250495)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GGGATTGTCATAGTAACATTT
- Hairpin Sequence:
-
5'-CCGG-GGGATTGTCATAGTAACATTT-CTCGAG-AAATGTTACTATGACAATCCC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGGGATTGTCATAGTAACATTTCTCGAGAAATGTTACTATGACAATCCCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGGGATTGTCATAGTAACATTTCTCGAGAAATGTTACTATGACAATCCC-3'
Other clones with same target sequence:
(none)
