Construct: shRNA TRCN0000250508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145601.1-139s21c1
DNA Barcode:
AGCCAGAAACAGAGTCGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cngb1 (333329)
Gene Description:
cyclic nucleotide gated channel beta 1
Transcript:
RefSeq NM_145601.1 (CURRENT)
Match location:
Position 139 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCAGAAACAGAGTCGAAAC
Hairpin Sequence:
5'-CCGG-AGCCAGAAACAGAGTCGAAAC-CTCGAG-GTTTCGACTCTGTTTCTGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCAGAAACAGAGTCGAAACCTCGAGGTTTCGACTCTGTTTCTGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCAGAAACAGAGTCGAAACCTCGAGGTTTCGACTCTGTTTCTGGCT-3'

Other clones with same target sequence:

(none)