Construct: shRNA TRCN0000250519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183026.1-111s21c1
DNA Barcode:
TGGCCGGTGTGCTGTACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Defb14 (244332)
Gene Description:
defensin beta 14
Transcript:
RefSeq NM_183026.1 (NON-CURRENT)
Match location:
Position 111 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCCGGTGTGCTGTACTTAA
Hairpin Sequence:
5'-CCGG-TGGCCGGTGTGCTGTACTTAA-CTCGAG-TTAAGTACAGCACACCGGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCCGGTGTGCTGTACTTAACTCGAGTTAAGTACAGCACACCGGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCCGGTGTGCTGTACTTAACTCGAGTTAAGTACAGCACACCGGCCA-3'

Other clones with same target sequence:

(none)