Construct: shRNA TRCN0000250560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028900.3-1983s21c1
DNA Barcode:
AGAGCTGAAGGACAAGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gcc1 (74375)
Gene Description:
golgi coiled coil 1
Transcript:
RefSeq NM_028900.3 (NON-CURRENT)
Match location:
Position 1983 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTGAAGGACAAGTATAT
Hairpin Sequence:
5'-CCGG-AGAGCTGAAGGACAAGTATAT-CTCGAG-ATATACTTGTCCTTCAGCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTGAAGGACAAGTATATCTCGAGATATACTTGTCCTTCAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTGAAGGACAAGTATATCTCGAGATATACTTGTCCTTCAGCTCT-3'

Other clones with same target sequence:

(none)