Construct: shRNA TRCN0000250588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175276.3-4262s21c1
DNA Barcode:
GATCGTCCATCGGAGGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fhod3 (225288)
Gene Description:
formin homology 2 domain containing 3
Transcript:
RefSeq NM_175276.3 (NON-CURRENT)
Match location:
Position 4262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGTCCATCGGAGGATAAT
Hairpin Sequence:
5'-CCGG-GATCGTCCATCGGAGGATAAT-CTCGAG-ATTATCCTCCGATGGACGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGTCCATCGGAGGATAATCTCGAGATTATCCTCCGATGGACGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGTCCATCGGAGGATAATCTCGAGATTATCCTCCGATGGACGATC-3'

Other clones with same target sequence:

(none)