Construct: shRNA TRCN0000250597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146128.5-1260s21c1
DNA Barcode:
CCGACACCAACTATGTCAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlgap4 (228836)
Gene Description:
discs, large homolog-associated protein 4 (Drosophila)
Transcript:
RefSeq NM_146128.5 (NON-CURRENT)
Match location:
Position 1260 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACACCAACTATGTCAAAC
Hairpin Sequence:
5'-CCGG-CCGACACCAACTATGTCAAAC-CTCGAG-GTTTGACATAGTTGGTGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACACCAACTATGTCAAACCTCGAGGTTTGACATAGTTGGTGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACACCAACTATGTCAAACCTCGAGGTTTGACATAGTTGGTGTCGG-3'

Other clones with same target sequence:

(none)