Construct: shRNA TRCN0000250624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019587.2-5630s21c1
DNA Barcode:
CAACCACATCCACAGGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxnb3 (140571)
Gene Description:
plexin B3
Transcript:
RefSeq NM_019587.2 (CURRENT)
Match location:
Position 5630 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCACATCCACAGGTATTA
Hairpin Sequence:
5'-CCGG-CAACCACATCCACAGGTATTA-CTCGAG-TAATACCTGTGGATGTGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCACATCCACAGGTATTACTCGAGTAATACCTGTGGATGTGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCACATCCACAGGTATTACTCGAGTAATACCTGTGGATGTGGTTG-3'

Other clones with same target sequence:

(none)