Construct: shRNA TRCN0000250748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028355.3-1384s21c1
DNA Barcode:
CCCACAATTGGACTGCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndc1 (72787)
Gene Description:
NDC1 transmembrane nucleoporin
Transcript:
RefSeq NM_028355.3 (CURRENT)
Match location:
Position 1384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACAATTGGACTGCTATTT
Hairpin Sequence:
5'-CCGG-CCCACAATTGGACTGCTATTT-CTCGAG-AAATAGCAGTCCAATTGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACAATTGGACTGCTATTTCTCGAGAAATAGCAGTCCAATTGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACAATTGGACTGCTATTTCTCGAGAAATAGCAGTCCAATTGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000217801