Construct: shRNA TRCN0000250754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172122.1-2332s21c1
DNA Barcode:
CCAGGACAAGCTGGAATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crocc (230872)
Gene Description:
ciliary rootlet coiled-coil, rootletin
Transcript:
RefSeq NM_172122.1 (NON-CURRENT)
Match location:
Position 2332 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGACAAGCTGGAATTAAA
Hairpin Sequence:
5'-CCGG-CCAGGACAAGCTGGAATTAAA-CTCGAG-TTTAATTCCAGCTTGTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGACAAGCTGGAATTAAACTCGAGTTTAATTCCAGCTTGTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGACAAGCTGGAATTAAACTCGAGTTTAATTCCAGCTTGTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000181354