Construct: shRNA TRCN0000250778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011734.3-924s21c1
DNA Barcode:
GAGAGTCCAATGCGGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Siae (22619)
Gene Description:
sialic acid acetylesterase
Transcript:
RefSeq NM_011734.3 (CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGTCCAATGCGGATTATA
Hairpin Sequence:
5'-CCGG-GAGAGTCCAATGCGGATTATA-CTCGAG-TATAATCCGCATTGGACTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGTCCAATGCGGATTATACTCGAGTATAATCCGCATTGGACTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGTCCAATGCGGATTATACTCGAGTATAATCCGCATTGGACTCTC-3'

Other clones with same target sequence:

(none)