Construct: shRNA TRCN0000250825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153409.4-2208s21c1
DNA Barcode:
TGCTGGGCTCATCGTTGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csrnp3 (77771)
Gene Description:
cysteine-serine-rich nuclear protein 3
Transcript:
RefSeq NM_153409.4 (NON-CURRENT)
Match location:
Position 2208 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGGGCTCATCGTTGTTTA
Hairpin Sequence:
5'-CCGG-TGCTGGGCTCATCGTTGTTTA-CTCGAG-TAAACAACGATGAGCCCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGGGCTCATCGTTGTTTACTCGAGTAAACAACGATGAGCCCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGGGCTCATCGTTGTTTACTCGAGTAAACAACGATGAGCCCAGCA-3'

Other clones with same target sequence:

(none)