Construct: shRNA TRCN0000250846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178664.4-1420s21c1
DNA Barcode:
GCTTTCAAGTCTGGCTATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B3gntl1 (210004)
Gene Description:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1
Transcript:
RefSeq NM_178664.4 (NON-CURRENT)
Match location:
Position 1420 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCAAGTCTGGCTATTAC
Hairpin Sequence:
5'-CCGG-GCTTTCAAGTCTGGCTATTAC-CTCGAG-GTAATAGCCAGACTTGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCAAGTCTGGCTATTACCTCGAGGTAATAGCCAGACTTGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCAAGTCTGGCTATTACCTCGAGGTAATAGCCAGACTTGAAAGC-3'

Other clones with same target sequence:

(none)