Construct: shRNA TRCN0000250894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040400.2-4470s21c1
DNA Barcode:
TTCGGAGGAGAAGGGTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tet2 (214133)
Gene Description:
tet methylcytosine dioxygenase 2
Transcript:
RefSeq NM_001040400.2 (CURRENT)
Match location:
Position 4470 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGGAGGAGAAGGGTCATAA
Hairpin Sequence:
5'-CCGG-TTCGGAGGAGAAGGGTCATAA-CTCGAG-TTATGACCCTTCTCCTCCGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGGAGGAGAAGGGTCATAACTCGAGTTATGACCCTTCTCCTCCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGGAGGAGAAGGGTCATAACTCGAGTTATGACCCTTCTCCTCCGAA-3'

Other clones with same target sequence:

(none)